靶向grp78基因rna干扰重组慢病毒载体及其构建方法

GRP78 gene targeted RNA interference recombinant lentivirus vectors and construction method

Abstract

本发明涉及靶向GRP78基因RNA干扰重组慢病毒载体的构建、筛选及其用途。本发明利用RNA干扰技术,针对GRP78基因的不同靶序列,构建了四个能在293细胞中表达siRNA的慢病毒真核表达载体,干扰慢病毒是由pLv‑GRP78shRNA、pMD2.G和pSPAX2载体共转染293T细胞培养获得。本发明靶向GRP78基因RNA干扰重组慢病毒载体能高效特异性的抑制GRP78基因表达,可用于制备治肿瘤相关性基因药物。
The invention relates to construction, screening and application of GRP78 (glucose regulated protein 78) gene targeted RNA interference recombinant lentivirus vectors. With use of an RNA interference technique, aiming at different target sequences of a GRP78 gene, four lentivirus eukaryotic expression vectors for expressing siRNA in 293 cells are constructed, an interference lentivirus is obtained through co-transfecting the 293 T cells with pLv-GRP78shRNA, pMD2.G and pSPAX2 and culturing. The GRP78 gene targeted RNA interference recombinant lentivirus vectors can efficiently and specifically inhibit the expression of the GRP78 gene, and can be used for preparing related gene medicines for treating tumor.

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