The invention relates to a fruit preservation box. The fruit preservation box comprises an upper box body and a lower box body which can be oppositely closed in a matching manner, wherein the rear sides of the upper box body and the lower box body are connected together through a connection part; a plurality of semi-spherical concave pit parts are symmetrically arranged on the upper box body and the lower box body; the concave pit parts are arranged in a hollowing manner; and at least one group of latching mechanisms is arranged on the front sides of the upper box body and the lower box body in a matching manner. The fruit preservation box is capable of fixing fruits in the oppositely-closed concave pit parts, and effectively preventing the condition of the movement of the fruits, thus collision among the fruits is avoided. Meanwhile, the concave pit parts are arranged in the hollowing manner, so as to avoid forcible extrusion of the concave pit parts for the irregular spherical fruits, thus protection for the fruits is further realized. In addition, the upper box body and the lower box body are connected together through the connection part, and are buckled by virtue of the latching mechanisms, and the operation is simple and fast, thus packaging efficiency for the fruits can be increased.