Reagent kit capable of simultaneously detecting monocytosis Listeria monocytogenes, bacillus cereus, cronobactersakazakii and staphylococcus aureus

一种同时检测单增李斯特菌、蜡样芽孢杆菌、阪崎克罗诺杆菌和金黄色葡萄球菌的试剂盒

Abstract

一种可同时检测单核细胞增多性李斯特菌、蜡样芽孢杆菌、阪崎克罗诺杆菌、金黄色葡萄球菌的一管多重扩增内标PCR试剂盒,该试剂盒包含:PCR反应预混液及对照品;检测方法是:首先采集样品,进行增菌培养,培养后提取DNA,制备PCR反应管,置于PCR仪上进行PCR扩增,得PCR扩增产物进行检测结果判定。优点是:该试剂盒一次反应可以同时快速检测上述四种细菌。与现有的检测方法相比,该方法使用方便、可靠性好、检测周期短、灵敏度高、特异性强、成本低廉、操作步骤简单,适用于高通量操作、标准化操作,而且可以指示因样品处理过程中存在聚合酶抑制因子及操作失误导致的假阴性结果,防止结果误判,有助于提高食品安全检测的水平及食源性疾病的预防和控制。
The invention discloses a one-tube multi-amplification internal-control PCR (polymerase chain reaction) reagent kit capable of simultaneously detecting monocytosis Listeria monocytogenes, bacillus cereus, cronobactersakazakii and staphylococcus aureus. The one-tube multi-amplification internal-control PCR reagent kit comprises a PCR premixed liquid and reference substances. A detection method includes acquiring samples; carrying out enrichment culture; extracting DNA (deoxyribonucleic acid) after enrichment culture is carried out; preparing PCR tubes; arranging the PCR tubes on PCR instruments and carrying out PCR amplification to obtain PCR amplification products; judging detection results of the PCR amplification products. The one-tube multi-amplification internal-control PCR reagent kit has the advantages that the monocytosis Listeria monocytogenes, the bacillus cereus, the cronobactersakazakii and the staphylococcus aureus can be simultaneously quickly detected by the aid of the one-tube multi-amplification internal-control PCR reagent kit by means of one-step reaction; the detection method is convenient to implement, good in reliability, short in detection period, high in sensitivity and specificity, low in cost and suitable for high-flux operation and standardized operation and includes simple operation steps as compared with existing detection methods; polymerase inhibiting factors and false negative results due to misoperation in sample treatment procedures can be indicated, accordingly, misjudgment on the results can be prevented, and the one-tube multi-amplification internal-control PCR reagent kit and the detection method are beneficial to improving the level of food safety detection and preventing and controlling foodborne diseases.

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