Method for increasing cell transfection ratio of superparamagnetic gene carrier through uniform magnetic field

均匀磁场提高超顺磁性载基因载体细胞转染率的方法

Abstract

The invention relates to a method for increasing the cell transfection ratio of a superparamagnetic gene carrier through a uniform magnetic field. The method comprises the following steps: adding a superparamagnetic carrier carrying genes into culture cell solution under the uniform magnetic field; vibrating slightly till the carrier is uniformly dispersed into the culture solution; putting a culture container under the uniform magnetic field; uniformly driving the superparamagnetic carrier carrying the genes onto the surfaces of cells (including adherent culture or suspension culture cells) in the culture container under the action of uniform magnetic field force, so that all cells in a system have equal opportunities and contact the superparamagnetic carrier carrying genes. By adopting the method, the carrier concentration of the genes is used at a minimum amount, and highest gene transfection efficiency is achieved; meanwhile, the toxicity of a high-concentration superparamagnetic gene carrier on the cells is lowered. The method has the advantages of easiness, convenience, safety, reusability and the like, and has a wide application prospect.
本发明涉及利用均匀磁场提高超顺磁性基因载体细胞转染率的方法,在均匀磁场下,可以使携带基因的超顺磁性载体,加入到培养细胞液中,经过轻微振动,均匀地分散在培养液中,将此培养容器置于均匀磁场下,在均匀磁场力的作用下,便能够将携带基因的超顺磁性载体,均匀驱使到培养容器中的细胞表面(包括贴壁培养的或悬浮培养的细胞),使体系中的所有的细胞均有同等的机会,接触到携带基因的超顺磁性载体,这样便能够最小量地使用基因的载体浓度,获得最大的基因转染效率,同时降低了高浓度超顺磁性基因载体对细胞的毒性。本发明具有简便、安全、可重复使用等优点,应用前景广阔。

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